【摘要】 据国内其它研究单位的报道及我们研究得知,柞蚕三种类型的软化病均属细菌所致.本试验从研究病、健蚕中肠内和柞叶表面的细菌种类及致病性着手,试图分析作蚕软化病发生原因.本试验应用22种不同种类培养基,在病、健蚕中肠及柞叶表面共分得227株需氧菌.经鉴定表明,三者之间菌株的种类及检出株数有明显不同.测定这些需氧菌株的致病性,只有七株较强,而且表现对稚蚕致病性强,壮蚕致病性弱.单一菌株的致病性与添菌量成正相关,单一菌株引起的病征多变.另外还表现出混合菌株添食致病性比单一菌株强.从而,我们认为:柞蚕软化病似乎是和蚕体内菌群失调以及在一定条件下蚕体与细菌之间相互作用有密切关系.更多还原

【Abstract】 It is known that the three types of flacheria of Antheraea pernyi are caused by bacteria according to previous reports and our studies. This experiment was to analyze the cause of flacheria of Chinese tusser through research on the strains of bacteria in the mid guts of flacheria infected and healthy tusser larvae and on the surface of oak leaves, and on their pathogenicity to the silkworm. 227 strains of aerobe were isolated by using 22 kinds of media, and obvious differences existed among the strains isolated from the above three different types. However, only 7 strains of them had stronger pathogenicity to the silkworm and it is stronger to young silkworms than to grown ones.The pathogenicity of a single strain had a positive corelationship with the amount of bacteria inoculated, the sympotoms caused by single strain were varied. Moreover the pathogenicity of mixed strains were stronger than that of the single.So it issupposed that the flacheria of Chinese tusser seemed to have close relation with the imbalance of bacteria groups in the larval body as well as with the interaction between larval body and bacteria strains in certain condition.更多还原