【摘要】 本工作用自旋标记ESR波谱方法研究了HEP.A22鼠腹水肝癌细胞和鼠正常肝细胞膜脂的运动性。采用两种脂肪酸类自旋标记化合物I(12,3)和I(1,14)。在0°—50℃的范围内,每隔5度记录ESR谱。测量了膜脂的旋转相关时间、序参数和摆动半幅度角,并计算了旋转活化能和相转变温度。结果表明:(1) 对癌细胞,其表层膜脂的流动性比正常细胞大,但在膜的深层,则结果相反;(2) 细胞癌变后,其动力学性质亦发生明显改变。在膜表层,癌细胞膜脂的相转变温度为～18℃,正常细胞为～30℃;而在膜的深层,正常细胞的Arrhenius作图表现为一直线,没有观察到转折点;对腹水肝癌细胞,Arrhenius作图不是一条直线,但不排斥多于一个转折点的可能性。更多还原

【Abstract】 The changes of cell membrane properties after carcinogenesis are important subjects. This paper reports the differences between the properties of HEP.A22 mouse ascites cell membrane lipids and those of the control cells. Two types of fatty acid spin labels Ⅰ(12,3) and Ⅰ(1,14) were used and the order parameters, rotational correlation times and half amplitude angle of wobbling for lipiid were determined. Arrhenius plots in range from 0℃ to 50℃ for both cells were done simultaneously. It was found that the order parameters of the cancer cell membraneare larger than those of the normal cell, but the wobbling half aplitude angle of fatty acid chain of cancer cell membrane is smaller than that of the normal cell in the depth of membrane; on the contrary at the surface of the membrane, the case is just the opposite. From the Arrhenius plots it was found that the phase transition temperature of the cancer and control cell membrane lipids are 18℃ and 30℃ respectively at the surface of the membrane, but in the depth of the membrane, the phase transition point is not found in range 0°—50℃ for both cancer and normal cells.更多还原