【摘要】 作者对大白菜(Brassica pekinensis,Rupr)雄性不育两用系可育株与不育株的全生育期进行了有关的生理生化比较分析,结果表明:DNA与RNA的含量在整个生育期两者之间无明显的差异;过氧化物酶及细胞色素氧化物酶同工酶在开花前两者之间没有明显的差异,但开花后,不育株的过氧化物酶同工酶活性明显地高于可育株,而细胞色素氧化酶同工酶酶谱则刚好相反;随着生育期的进行,可育株中蛋白质种类逐渐多于不育株,特别是在开花期表现更为突出,脯氨酸的含量在开花前两者的叶片中无明显的差异,但在开花期,可育株的花苞中含量高于不育株,然而这时在叶片中仍无差异;糖分的含量在初期两者无明显的差异,接近抽薹期直到开花期,不育株中非还原糖的含量低于可育株,而淀粉酶的活性又高于可育株;呼吸强度是开花前可育株高于不育株,开花后则相反;而抗氰呼吸在开花前两者之间无差异,随着生育期的发育,不育株的抗氰呼吸有增高的趋势,但在开花期的花苞中其抗氰呼吸强度急剧下降,而可育株的花苞中的抗氰呼吸强度逐渐升高,结果导致两者之间的差异极显著。更多还原

【Abstract】 An investigation was made on the physiological and biochemical cha-racteristics of the male sterile amphy-line of Brassica pekinensis. The plants investigated on were reaped in the preceding year. The experiment began with the sprouting of three leaves and ended at the flowering phase.A Comparative analysis showed:1. There was no evident difference between the content of DNA and RNA of the male fertile plants and that of the male sterile ones;2. In the flowering phase, the activity of cytochrome oxidase isozyme in the male sterile plants was lower than that of the male fertile ones, the activity of peroxidase isozyme, however, was higher than that of the fertile plants;3. The groups of proteins in the male fertile plants were obviously more than that in the male sterile plants. The content of proline in the floral organ of male fertile plants was higher than that of the male ste-rile ones. In the leaf blades, however, there was no such differences;4. The content of nonreducing sugar in the sterile plants became lower than that in the fertile ones nearly flowering phase. The activity of am-ylase in the sterile plants, however, became higher than that in the fer-tile ones;5. The respiratory intensity of fertile plants was higher than that of sterile plants before flowering phase, but the contrary is the case after flowering phase; there was no observable difference in CN-resistant res-pirations between male fertile and male sterile plants before flowering ph-ase, but there was difference after flowering phase 。更多还原