【摘要】 <正> 近十年来由于细胞松弛素B(Cyto-chalasin B,以下简称CB)引起细胞群体去核的方法的建立和不断完善,为细胞核与质的相互作用关系研究提供了一个新的实验手段。为详尽地了解CB处理细胞所获的核体和胞质体的特性,以便利用其进行核质关系方面的研究,我们利用显微与电镜放射自显影技术对CB处理所获各类核体作了RNA代谢的研究。核体(Karyoplast)是外面包被着细胞质膜并带有不同量胞质的细胞核,核体本身就是研究细胞核与质相互关系的好材料。通过对核体的研究,可从中探讨大分更多还原

【Abstract】 Karyoplasts from IBRS-2 cellwith various volume of cytoplasm wereprepared by treatment with cytochala-sin B (10μg/ml) and colchicine (0.4μg/ml) and then scouring with Hankssolution.Karyoplast’s structure and ability to synthesize RNA were studiedby means of light and electron mi-croscopic autoradiography. Most of karyoplasts contained anormal of nucleus enclosed by plasmamembrane with various volume ofcytoplasm.Karyoplasts were able tosynthesize RNA after adhesion andspreading on the glass surface uponcondition of static cultivation,whereasno adhesion and RNA synthesis couldbe found in suspended cultivation. Nucleus free of cytoplasm andplasma membrane (naked nucleus) prepared by homogenizing IBRS-2 cellswere not able to adhere and synthesizeRNA in static cultivation.更多还原