【摘要】 <正> 近年来应用发光细菌的发光系统来检测各种物质的报道日见增多,尤其在检测环境污染、有毒物质等方面应用较多。由于其快速易行,灵敏度高而日益受到重视。但在抗菌素效价的检测上,则尚未见报道。本文主要利用发光细菌的发光特性易受外界环境因素影响的特点,选用目前应用很广的几种抗菌素——四环素,多粘菌素、红霉素、卡那霉素和头孢霉素对发光细菌更多还原

【Abstract】 Effects of five antibiotics （tetracycline, polymyxinB, erythromyxin, kanamycin and cephalosporin on luminescence of Photobacterium phosphoreum A₂） were reported in this paper. Each of them was added separately to bacterial liquid culture. After a period of incubation, the light intensities were measured. The lowest concentration of these antibiotics which completely inhibiting bacterial luminescence were as follows: tetracycline 0. 2ug/ml; polymyxinB 2ug/ml; erythromycin 25ug/ml; kanamycin 40ug/ ml; and cephalosporin 200ug/ml. If antibiotics were added to cultures already emitting light and suspened in phos- phorous buffer solutions, all the above antibiotics gredually depressed the light inten- sities emitted by bacteria. Linear relations were obtained by plotting the concentra- tion of these antibiotics （polymyxinB, cephalosporin and kanamycin） against the rela tive light intensity. Thus, unknouw concentrations of these antibiotics can be accur- ately estimated by using these standard lines. The test might be completed within an hour. Only bacterial cells in lag phase were yery sensitive to antibiotics. For best results the concentration of suspended cells had to be 10⁷--10⁸ cells/ml.更多还原