【摘要】 用小鼠骨髓瘤细胞(Sp 2/0) 与HBsAg免疫过的BALB/C小鼠脾细胞融合,获得11株产生抗-HBs的杂交瘤细胞系,其中10株分泌抗-a决定簇的MCA (单克隆抗体),用ID法测得对adr、adw和ayw亚型滴度可达1/100～1/3,200。另一株分泌抗-d决定簇的MCA,对adr和adw亚型ID法滴度为1:1,6004～1:3,200,而对ayw则不起反应。对其中的5株细胞分泌的MCA免疫球蛋白进行亚类分析,证明一株为IgG2a,4株为IgG1。更多还原

【Abstract】 We have developed eleven monoclonal antibodies (MCA) generated against purified hepatitis B antigen (HBsAg). Among them, ten of these MCA showed specificity against the group-specifie-a,determinant of HBsAg, and only one demonstrated antibody activity against the ’d’ determinant.The titers of 10 anti-a MCA against adr, adw and ayw subtypes varied between 1/100-1/3200 and that of anti-d against adr and adw subtypes ranged from 1/1600-1/3200 when tested by ID:Five of these MCA have been tested for IgG subclasses. And we found that one was identified to be IgG2a, all the others were IgG1.更多还原