【摘要】 本文以青花菜的不同部位的组织作为外植体,培养于MS培养基中,补充以不同浓度的BA及NAA,均能产生愈伤组织。 无论叶片、中肋及花序柄作为外植体,所分化的根的长度均随NAA浓度的减少而逐渐增加(BA均为5毫克/升),而分化的根数、则逐渐减少。用中肋及花序柄培养的都比叶片培养的根长,根数多。 芽的分化比根的分化少得多,但用花序柄为外植体,在BA 5毫克/升及NAA为0.1毫克/升的培养基中,可分化很多的芽,发根亦较长,是青花菜组织培养获得采种植株的适宜材料及适宜的激素配合。更多还原

【Abstract】 Different plant parts of broccoli ( Brassica oleracea var. italics Plenck ) were excised and cultured on the Murashige-Skoog ( MS ) media supplemented with various combinations of benzyadenine ( BA ) and naphthaleneacetic acid ( NAA ) .All the explants excised from the lamina, leaf-midrib and inflorescence stem tissues were able to form callus. The length of the roots differentiated from callus increased as the NAA concentration decreased, but the reverse was true for the number of roots.The number of buds differenceded form each explant was much less than that of the number of roots. The explant of inflorescence stem cultured on the MS medium supplemented with BA 5mg/1 and NAA 0.1mg/1 was found to be the suitable material and suitable hormone combination to obtain plants for seed production.更多还原