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应用酶联免疫吸附试验检测抗肿瘤i-RNA免疫活性初步研究

PRELIMINARY STUDY ON DETERMINATION OF ANTI-TUMOR IMMUNE RNA WITH ELISA

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【作者】 金壮杨明久

【Author】 Jin Zhuang Yang Mingjiu Department of Immunology

【机构】 中国医科大学基础部免疫研究室中国医科大学基础部免疫研究室

【摘要】 <正> 从免疫动物的淋巴组织提取的iRNA能在受者动物体内诱导免疫记忆和制造抗体,这已被许多实验室所证实。但是iRNA在动物体内诱发的血清抗体水平较低,用不敏感的实验方法难以准确测定。ELISA是七十年代发展起来的测定抗体的新

【Abstract】 An Enzyme Linked Immunosorbent Assay was used to detect the level of IgG formation in rabbits induced by hemologeous antitumor immune RNA. Being compared with other methods to test the humoral activity induced by immune RNA, the advantage of ELLSA is high sensitive, simple and easy, it can be used to test the level of IgG induced directly.by immune RNAIt was shown that immune RNA from the Spleen and liver of a rabbit immunized with Hela cells can transfer the ability of antibody formation to a normal rabbit recipient.Our results show also that when rabbits were administrated continuously by injection of immune RNA into peritoneal cavity (2mg/day, 3days) , they may be induced to product specific IgG antibody in sera, but the titre of IgG is not higher. However, one time adminitration of iRNA, though the total amount is the same, can not lead to the production of specific IgG antibody.

  • 【文献出处】 中国医科大学学报 ,Journal of China Medical University , 编辑部邮箱 ,1983年01期
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