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烟草花叶病毒几个分离物的比较研究

COMPARATIVE STUDIES ON SOME ISOLATES OF TOBACCO MOSAIC VIRUSES

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【作者】 郭惠民黄素珍朱孔生郑礼骆学海王鸣岐

【Author】 GUO HUI-MIN HUANG SU-ZHEN ZHU KONG-SHENG ZHENG LI LUO XUE-HAI WANG MIN-QI (Biological Department of Fudan University,Shanghai.) Hua-nan Agricultutal Institute.

【机构】 上海复旦大学生物系华南农学院植保系上海复旦大学生物系

【摘要】 本文从血清学关系、外壳蛋白亚基分子量、氨基酸组成和放射性碘标记酶解肽谱等方面比较研究了广州和上海地区的烟草、茄子、辣椒、番茄上分离到的烟草花叶病毒几个分离株。试验结果指出,它们与烟草花叶病毒普通株(TMV_c),长叶车前花叶病上海株(RMV_gh)都有所不同。并对放射性碘标记酶解肽谱在病毒诊断鉴定上的应用进行了讨论。

【Abstract】 Four isolates of tobacco mosaic virus (TMV) were purified from the egg-plant,pepper,tomato and tobacco plant growing in the suburb of Shang-hai and Guang-Zhou. The results of the comparative studies on the relationships of the se-rology,molecular wight of coat pro-tein sub-unit,amino acid composi-tion as well as 125I-labled peptide mapping of coat protein showed thatthe four isolates are the "member" or "strains" of the tobomoviruses with different 125I-peptide mapping of the coat protein from that of the common strain of TMV and that of the Shang-hai strain of ribgrass mosaic virus. The autoradiogram of the peptide mapping of the coat protein is useful for the identification of the "member" or "strain" of TMV.

  • 【文献出处】 实验生物学报 ,Acta Biologiae Experimentalis Sinica , 编辑部邮箱 ,1983年01期
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