【摘要】 本文以肠系膜作为天然载物,于扫描电子显微镜下研究其表面自由细胞。腹腔自由细胞如多形核白细胞、巨噬细胞、淋巴细胞的形态特点,特别是注射到腹腔里的靶细胞(胚肝细胞)和免疫反应细胞之间的关系均清晰可见。多形核白细胞侵蚀靶细胞及巨噬细胞致靶细胞肿胀和溶解。实验亦观察到免疫细胞间的协同作用及乳斑增多。更多还原

【Abstract】 This paper reports a simple method using the mesentery as a naturai mountingsubstrate for the study of mesenteric free cells under the scanning electron micros-cope.Mouse mesentery was cut into pieces about Ⅰ square centimeter in size andadhered on to neutral filter paper.The specimen were fixed in 5% glutaraldehyde and1% osmium tetroxide for 20 minutes,each step washed lightly with pH 7.4 PBS,dehydrated with ascending series of alcohol,put into amyl acetate,critical pointdried with CO₂,coated with gold and examined in scanning electron microscope.The morphologic features of free cells in the peritoneal lumen,such as polymorpho-nuclear leucocytes,macrophages and lymphocytes could be observed satisfactorily.We studied in particular the relationship between immunoreactive cells and targetcells（fetal liver cell）injected into the peritoneal cavity,Polymorphonuclear leuco-cytes could be seen invading into and eroding the target cells and macrophage cau-sing their swelling and lysis.Cooperative action of immunoreactive cells and anincrease in number of milk spots were also observed.更多还原