【摘要】 用登革4型H241株病毒免疫的小鼠脾细胞与小鼠骨髓瘤细胞融合,获得了5个产生抗登革4型病毒单克隆抗体的杂交瘤细胞系。这些杂交瘤细胞的上清液及小鼠腹水抗体均用间接免疫荧光法检测。其中两个杂交瘤细胞系产生的抗体对登革4型病毒具有型特异性,它们的荧光抗体滴度分别为1:10,240～1:20,480和1:2,560。另两个细胞系产生的抗体对登革2型病毒有交叉;另1个对登革1型病毒有交叉。更多还原

【Abstract】 Monoclonal antibodies against H241 strain of dengue-4 virus were obtained from lymphocyte hybri-domas produced by fusing immune mouse lymphocytes with mouse myeloma cells. Hybridoma cell culture supernatants were screened by indirect immunofluorescent antibody technique. Monoclonal antibodies in mouse ascitic fluid induced by five selected hybridomas were characterized also by the immuno-fluorescence test. Two hybridoma-producing antibodies were type-specific for dengue-4. Their titers were 1:10,240-1:20,480 and 1:2,560 respectively. The other two hybridoma antibodies were cross-reactive with dengue-2 virus. Another preparation was cross-reactive with dengue-1 virus.更多还原