【摘要】 在三分三细胞悬浮培养中,用无离子水代替重蒸馏水,培养细胞的干重增加和培养细胞中莨菪碱、东莨菪碱的含量稍有降低(分别降低1.2％和2.9％)。但无离子水可大大降低电费等成本(约降低1030元/吨培养基),且容易大量制备适应未来的工业生产。用市售白糖代替蔗糖,培养细胞的干重稍增加,两种生物碱的含量降低不大(约5.4％)。但市售白糖可大大减少用量(50％)和降低成本(88％)。三分三悬浮细胞中含有丰富的淀粉酶(平均酶活性约为4.37毫克还原糖/克鲜重/小时),所以它能利用淀粉作为唯一碳源而生长。更多还原

【Abstract】 Both increased dry wt. of the culture cells and contents of hyoscyamine and scopolamine in the culture cells were decreased slightly (decreased about 1.2% and 2.9% respectively) when non-ionic water was used instead of redistilled water in the cell suspension culture of Anisodus acutangulus. But the non-ionic water could reduce costs largely, e. g. power cost etc. (can be reduced about 1030 yuan/tonne medium). Since non-ionic water can now be mass-produced easily, it suits the needs of industrial production in the future. The increased dry wt. of culture cells was a litter high and contents of two alkaloids was decreased slightly (about 5.4%) in the cell suspension culture of A. acutangulus when store confectioner’s sugar was used instead of sucrose. But the applied quantities of store confectioner’s sugar and costs could be reduced 50% and 88% respectively. The suspension cells of A. acutangulus contain rich am- ylase (average amylase activity was 4.37mg reducing sugar/g, fresh wt./hour), so it could use starch as their only carbon source for the growth.更多还原