【摘要】 本文研究了类淋巴母细胞干扰素的诱导特性:Namalwa细胞系的干扰素诱生能力大于Raji和余奴系,而诱生剂中以NDV和仙台病毒诱生干扰素的能力较大,但“起动”和超诱导均无作用;诱生干扰素适宜的细胞浓度为10~6/毫升;增殖期的Namalwa细胞诱生能力较大;黄芪和5’-溴脱氧尿嘧啶核苷对Namalwa细胞干扰素的诱生系统有明显刺激作用,在最适条件下粗制干扰素平均滴度可达40,000单位/毫升。更多还原

【Abstract】 In the present paper, the induction characteristic of lymphoblastoid, in-terferon in vitro is described. Results indicate that Namalwa cell line possesses a higher interferon-inducing ability than Raji and Yu-Nu cell lines. NDV B1 strain, F strain and Sendai virus can induce more interferon than BB1 virus and poly 1 : C. Priming of pretreating cells with homologous interferon or superinduction did not effect interferon production. The optimal cell concentration for interferon induction is about 106 cells/ml. More interferon can be induced in cell cultures during the proliferating phase. Namalwa cells pretreated with Astragalus membranaceus are found to be able to induce more interferon than those untreated cells. 5’-bromdeoxyuridine can significantly stimulate Namalwa cell interferon induction. Under the optimal conditions, the mean titer of crude interferon is about 4×104 units /ml. These results may offer some evidences to explain the interferon induction mechanism of Namalwa cell and also a technical method for large scale production of Namalwa cell interferon,更多还原