【摘要】 将β-蜕皮激素加入小麦花药培养基中,观察到它对花粉苗诱导的影响。0.5～10毫克/升β-蜕皮激素提高了花粉胚和愈伤组织诱导率,它们能直接长成小苗。1～10毫克/升激动素抑制这种作用。β-蜕皮激素加2,4-D能得到高的愈伤组织诱导率,但这些愈伤组织不能在原培养基上直接分化成苗。β-蜕皮激素1毫克/升与萘乙酸1～5毫克/升相配合产生最高的花粉苗诱导率。这些小苗移入土中容易成活。对花药培养中花粉发育过程进行了细胞学观察。更多还原

【Abstract】 β-Ecdysone was incorporated into medium for anther culture of wheat, and its influences on induction of pollen plantlets had been observed. β-ecdysone at 0.5-10 mg/l increases the induction-frequency of pollen embryoids and callus which can grow directly into plantlets. Kinetin at 1-10 mg/l inhibits this effect. β-ecdysone plus 2,4-D give a higher induction-frequency of callus which, however, can not differentiate directly on this medium. The combination of β-ecdysone at 1 mg/l with NAA at 1-5 mg/l gives the highest induction-frequency of pollen plantlets. These plantlets have a higher viability when planted in soil. Cytological observations of pollen development in anther culture were made.更多还原