【摘要】 <正> 1979年1～5月进行婴幼儿毛细支气管炎病毒分离,同时采取患儿血清,用免疫酶法及中和试验检测其中呼吸道合胞病毒的抗体,试图从血清学方面对长沙地区流行的婴幼儿毛细支气管炎病因加以证实。材料和方法一、中和试验将患儿血清（包括双份血清与单份血清）从1:4开始作倍比稀释,分别与呼吸道合胞病毒Long·株(100TCD₅₀/0.1毫升)等量混合,置37℃作用1小时,各接种3支Hela细胞管,每管种入0.2毫升,每批实验均设病毒对照、血清对照、正常细胞对照,然后将全部实验管置33℃下培养,以第8日观察的结果为判定标更多还原

【Abstract】 In this paper, the application of immunoenzyme method(solid phase enzyme immunoassay) and neutralization test to respiratory syncytial virus (RSV) antibody in infants with bronchiolitis was described. For etiological study, the sera of 43 cases of bronchiolitis in infants were examined by serologic methods with lmmunoenzyme method and neutralization test (among these cases 15 were paired sera). As a result of this investigation, RSV was found again to be the most frequent etiological agent of bronchiolitis in infants in Changsha area.更多还原