【摘要】 本文报道了玉米花药培养的主要结果。品种之间诱导频率差异很显著。接种花药时,花粉单核中期诱导频率最高。培养基中加入500毫克/升酪朊水解物,0.5％活性炭,15％蔗糖能显著提高花粉胚状体和愈伤组织的诱导频率。在雄核发育的研究中,观察到有营养细胞发育途径,生殖细胞发育途径,营养细胞和生殖细胞混合发育途径,以及均等分裂发育途径等四种类型,并对这四种途径进行了讨论。更多还原

【Abstract】 The present paper deals with the main experimental results about the anther cul- ture in maize.Induction frequency varies with the different varieties and it is the highest when the excised anthers with the pollen grains at the middle-uninuclear stage are inoculated. Induction frcguency of pollen embryoids or calli increases apparently when the medium is added with 500 mg/l casein hydrolysate,0.5% active carbon and 15% sucrose. Four different pathways of androgenesis have been observed:Pathway of vegetative cell,pathway of generative cell,pathway of vegetative and generative cell in mixed development and pathway of equal division of the uninuclear pollen grain.All these pathway of development have been discussed.更多还原