【摘要】 <正> 为了阐明烧伤愈合过程中的组织再生及其分化的机制,我们曾观察了豚鼠皮肤烧伤愈合过程中的糖元、核酸、碱性磷酸酶及SH-基的改变,至于脱氢酶在这方面有何变化,了解的非常不够。就现有的资料看来,只有少量关于琥珀酸脱氢酶(SDH)的报导,而更多还原

【Abstract】 Necrotic burn was made on a definite area of the dorsal skin of 13 adult maleguinea pigs with circulating hot water of 80℃ in a specially designed apparatus. Theskin specimens were taken at definite time intervals and both the healthy and the burnedskin areas were studied with the histochemical methods modified from those of Scarpelliet al and Natochin for α-ketoglutarate (KDH), succinate (SDH), malate (MDH), lactate(LDH) and glutamate (GDH) dehydrogenases. In the healthy skin, except the fibers of the derma and the cornified cpithelial ele-ments, these five enzymes were demonstrated with their special patterns. According totheir reactive intensity in different parts, such as in epidermis, the order from the moreto the less was LDH, MDH, GDH, SDH, and KDH. In addition, these enzymesshowed a similar gradient distribution in epidermis. The strongest enzyme activity wasfound in the lower part of the Malpighian layer. It faced gradually upwards, and dis-appcared completely in the stratum corneum. On the first day after the burn, the above-mentioned enzymes were disappeared inthe burned area, while the activities of these enzymes in the healthy area were unchanged.On the 3rd--13th day after the burn, the marginal epidermal layer was increased inthickness. The more the epidermis neared to the burned area, the higher was its thick-ness. In front of the thickest epidermis, there was a thin sheet of migrating epidermalcells. The distribution and reactive intensity of these enzymes in the thicker epidermiswere similar to that found in the Malpighian layer of the healthy skin. Toward the shcet-form epidermis, the activities of these enzymes were decreased gradually. In comparison with the healthy dermis, the granulation tissue revcaled a higheractivity of the five enzymes. The significance of the enzymes present was discusscd.更多还原