【摘要】 目的 探究血清丙酮酸激酶M2(PKM2)、瞬时受体电位通道C6(TRPC6)水平与肺炎支原体（MP）感染患儿病情进展及预后的相关性，为临床诊疗提供参考依据。方法 选取2024年1月-2025年1月在首都医科大学附属首都儿童医学中心呼吸科确诊的104例MP感染患儿作为研究组，另选取同期106名健康体检儿童为对照组。根据病情评估结果，将研究组分为轻度组62例，重度组42例。根据预后评估结果，将研究组分为预后良好组68例，预后不良组36例。采用ELISA法检测MP患儿血清PKM2、TRPC6水平；使用多因素logistic分析MP感染患儿预后不良的风险因素；采用受试者工作特征（ROC）曲线分析血清PKM2、TRPC6水平对MP感染患儿预后不良的预测价值。结果 与对照组比较，研究组血清PKM2、TRPC6水平升高，差异均有统计学意义（t=43.862、41.886,P均<0.05）。与轻度组比较，重度组血清PKM2、TRPC6水平提高，差异均有统计学意义（t=6.736、6.697,P均<0.05）。与预后良好组比较，预后不良组血清PKM2、TRPC6、CRP水平和CPIS评分明显升高，差异均有统计学意义（t=7.001、7.006、3.093、9.278,P均<0.05）。血清PKM2、TRPC6水平和CPIS评分升高是MP感染患儿预后不良的独立危险因素（P均<0.05）。血清PKM2、TRPC6水平联合预测MP感染患儿预后不良的曲线下面积（AUC）为0.948，联合预测优于单独预测（Z=3.431、3.263、3.106,P均<0.05）。结论 MP感染患儿血清PKM2、TRPC6水平升高，与患儿病情进展及预后相关，二者联合预测MP感染患儿预后不良具有一定价值。更多还原

【Abstract】 Objective To discuss the correlation between serum pyruvate kinase M2(PKM2) and transient receptor potential cation channel subfamily C member 6(TRPC6) and the progression and prognosis of Mycoplasma pneumoniae(MP) infection in children. Methods From January 2024 to January 2025, 104 children diagnosed with MP infection in the Capital Center for Children’s Health,Capital Medical University were selected as the study group. Another 106 healthy children who passed the health examination test in our hospital during the same period and at same age group were served as the control group. Complying with the evaluation results of the condition, the study group was classified into a mild group of 62 cases and a severe group of 42 cases. Complying with the prognosis evaluation results, the study group was classified into a good prognosis group of 68 cases and a poor prognosis group of 36 cases. ELISA method was used to detect serum PKM2 and TRPC6 in patients. The risk factors for poor prognosis in children with MP infection were explored using multivariate logistic regression. Receiver operating characteristic(ROC) curve was used to explore the predictive value of serum PKM2 and TRPC6 for poor prognosis in children with MP infection. Results Compared with the control group, the study group had conspicuously higher serum PKM2 and TRPC6 levels(t=43.862,41.886; all P<0.05). Compared with the mild group, the severe group had conspicuously higher serum PKM2 and TRPC6 levels(t=6.736,6.697; all P<0.05). Compared with the good prognosis group, the poor prognosis group had conspicuously higher serum PKM2, TRPC6 and CRP levels, and CPIS score(t=7.001,7.006,3.093,9.278;all P<0.05). Elevated serum PKM2 and TRPC6 levels, and improved CPIS score were independent risk factors for poor prognosis in children with MP infection(P<0.05). The joint of serum PKM2 and TRPC6 levels had an AUC of 0.948 for predicting poor prognosis in children with MP infection; the joint prediction was superior to individual prediction(Z=3.431, 3.263, 3.106; all P<0.05). Conclusions Serum PKM2 and TRPC6 levels were elevated in children with MP infection, and were related to the progression and prognosis of the patient’s condition. The joint of the two had certain value in predicting poor prognosis in children with MP infection.更多还原