【摘要】 目的观察饮用苗药天门冬水煎剂72周的SD雄性大鼠学习记忆、空间探索能力及血清炎症因子水平、脑组织神经酶活性。方法 18只3周大鼠分为天门冬组、衰老组、对照组各6只,天门冬组大鼠自由饮用12 g/L的天门冬水煎剂72周(根据医用实验动物学72周相当于50~55岁,为大鼠老年期),衰老组大鼠自由饮用自来水72周,对照组大鼠自由饮用自来水6周。采用Morris水迷宫实验评价大鼠学习记忆能力及空间探索能力,采用酶联免疫法检测血清IL-1、IL-6及脑组织乙酰胆碱酯酶(Ach E)、乙酰胆碱转移酶(Ch AT)活性。结果与对照组比较,天门冬组和衰老组实验第1~4天大鼠水迷宫潜伏时间长(P均<0.05);与衰老组比较,天门冬组实验第4天大鼠水迷宫潜伏时间短(P<0.05)。与对照组比较,天门冬组和衰老组实验第2~4天游泳距离长(P均<0.05)。与对照组比较,天门冬组实验第5天首次穿越平台时间长、穿越平台次数少,衰老组实验第5天首次穿越平台时间长、穿越平台次数少、穿越平台区时间短(P均<0.05);与衰老组比较,天门冬组实验第5天首次穿越平台时间短、穿越平台次数多(P均<0.05)。与对照组比较,天门冬组和衰老组血清IL-1、IL-6水平升高(P均<0.05);与衰老组比较,天门冬组血清IL-1、IL-6水平降低(P均<0.05)。与对照组比较,天门冬组和衰老组Ach E活性增强,Ch AT活性减弱(P均<0.05);与衰老组比较,天门冬组Ach E活性减弱,Ch AT活性增强(P均<0.05)。结论饮用苗药天门冬水煎剂72周的SD雄性大鼠学习记忆及空间探索能力、血清炎症因子水平、脑组织神经酶活性得到改善。更多还原

【Abstract】 Objective To observe the learning and memory ability and spatial exploration ability,serum inflammatory factors,and brain tissue neuroenzyme activity of SD male rats after drinking 72-week Miao medicine asparagus decoction.Methods Eighteen rats were divided into the asparagus group,aging group,and control group with 6 rats in each group.Rats in the asparagus group were free to drink 12 g/L of asparagus decoction for 72 weeks( according to 72 weeks of medical laboratory zoology,which is equivalent to 50 to 55 years old,the old age of rats),the rats in the aging group were free to drink water for 72 weeks,rats in the control group were free to drink water for 6 weeks. The Morris water maze test was used to evaluate the learning and memory and space exploration ability in rats. Using enzyme-linked immunosorbent assay to detect the serum IL-1,IL-6,and acetylcholinesterase in brain tissue( Ach E) and choline acetyltransferase( Ch AT) activity. Results Compared with the control group,on day 1-4,the water maze latency of rats of the asparagus group and aging group was long( P < 0. 05). Compared with the aging group,on the 4 th day,the water maze latency of rats was short in the asparagus group( P < 0. 05). Compared with the control group,on day 2-4,the swimming distance of rats in the asparagus group and aging group was longer( P < 0. 05). On day 5,compared with the control group,the time of first crossing the platform of the rats in the asparagus group was longer,and the number of crossing platform was less; the time of first crossing the platform in the aging group was longer,and the number of crossing platform was less,with a shorter time of crossing the platform( all P < 0. 05). Compared with the aging group,on the fifth day,the time of first crossing the platform of the rats in the asparagus group was shorter,and the number of crossing platform was more( all P < 0. 05). Compared with the control group,the levels of serum IL-1 and IL-6 in the asparagus and aging groups were increased( both P< 0. 05). Compared with the aging group,the levels of serum IL-1 and IL-6 in the asparagus group were lower( both P <0. 05). Compared with the control group,the activities of Ach E in the asparagus group and aging group were enhanced,and Ch AT activity was decreased( both P < 0. 05). Compared with the aging group,the activity of Ach E was weakened and the Ch AT activity was increased in the asparagus group( both P < 0. 05). Conclusion The learning and memory and space exploration ability,the level of serum inflammatory factors,and brain tissue neuroenzyme activity of SD male rats after drinking 72-week Miao medicine asparagus decoction are improved.更多还原