【摘要】 酶法降解偶氮染料刚果红是一个复杂的过程,受温度、pH、酶量、刚果红浓度和双氧水浓度显著影响。为研究各因素及因素间交互作用对刚果红降解影响,提高刚果红的降解率,分别使用单因素法和响应面分析法对刚果红降解条件进行了优化。单因素实验结果显示灰盖鬼伞过氧化物酶降解刚果红的最适条件为:pH 5.0、32℃、酶量4.98 U、双氧水0.1mmol/L、刚果红20 mg/L,此时刚果红最高降解率为34.84%。然后选双氧水浓度、刚果红浓度和灰盖鬼伞过氧化物酶量作为3个因素,通过中心组合设计实验,用响应面法对刚果红降解进行优化分析,最后得到一个拟合度良好的二次多项方程模型(R2=0.9900)。方差分析结果显示,刚果红浓度和酶量是影响最显著的因素,双氧水与酶以及染料与酶之间的交互作用极显著。响应面分析优化后的反应体系为:双氧水浓度0.15 mmol/L,刚果红浓度为27.21 mg/L,酶为2.0 7 U,在此条件下,刚果红降解率达58.13%。更多还原

【Abstract】 Enzymatic degradation of the azo dye,Congo red by Coprinus cinereus peroxidease( CIP) is a complex process,which is greatly affected by pH,temperature,enzyme activity,the concentration of hydrogen peroxide( H 2 O 2) and dye. This work aimed to study these factors and to investigate the combined interactions between them by applying conventional one-factor-at-a-time( OFAT) method and response surface methodology( RSM) for degradation of Congo red with mutant CIP. Through the OFAT method the optimum conditions for degradation of Congo red were 32℃,pH 5. 0,0. 1 mmol / L of H 2 O 2,20 mg/L of Congo red and 4. 98 U CIP. Under these conditions,the degradation rate reached 34. 84%. Moreover,in order to optimize the reaction conditions of degradation system,the H 2 O 2 concentration,the CIP activity and the congo red concentration were selected as three factors for systematical analysis. Response surface analysis was conducted through central composite design and a reduced cubic model with a good fit of regression equation( R2= 0. 9900) was generated. ANOVA analysis of this model indicated that the Congo red concentration,the CIP activity,their interaction and the combined interaction between H 2 O 2 and CIP were the most significant on degradation of Congo red. The optimum conditions through RSM were found to be initial Congo red concentration of 27. 21 mg / L,2. 0 7 U CIP,0. 15 mmol / L of H 2 O 2,for the maximum degradation of 58. 13%.更多还原