【摘要】 以水热法合成的碲化镉(CdTe)纳米晶标记抗荧蒽抗体后,标记复合物的荧光强度增强,其分散性和稳定性良好。将此标记物用于直接竞争荧光免疫分析,测定了环境水样中荧蒽的含量。结果表明,在0.1～1000μg/L范围内有良好的线性关系;抑制率IC50为12.4μg/L,检出限IC20为13.1ng/L。对水样进行加标回收实验,回收率在95.1%～111%之间;相对标准偏差小于9%。本方法准确可靠,结果满意,能够满足环境中微量环境激素类污染物的检测需要。更多还原

【Abstract】 The labeled compounds,CdTe was combined with anti-fluoranthene antibody,had good dispersion and stability with the fluorescence intensity enhancing. A direct competitive fluorescent immunoassay with CdTe-anti-fluoranthene antibody to detect fluoranthene in water sample in the environment was developed. The result showed that fluoranthene can be determined in the concentration range from 0. 1 μg/L to 1000 μg/L with a correlation coefficient of 0. 9983,a sensitivity of ( IC50) of 12. 4 μg/L and a detection limit ( IC20) of 13. 1 ng/L. Trace environmental pollutant in environmental water samples were successfully determined with a good accuracy and suitability. The recovery was between 95. 1% and 111. 0% ,with relative standard deviation less than 9% .更多还原