【摘要】 分别制备了源于鸡、鹅、鸽、鹌鹑、孔雀、画眉鸟、珍珠鸡的禽型副粘病毒(APMV-1)7个强毒分离毒株和商品弱毒疫苗株克隆30(C30)的灭活油乳剂苗,并用这8种灭活油乳剂苗和C30株的活疫苗分别在鸡、鹌鹑、鹅和鸽进行了免疫及交叉攻毒保护试验。免疫后(PI)分别测定试验禽血清的新城疫病毒(NDV)血凝抑制(HI)抗体的滴度,并于PI 5周用强毒株进行攻毒。结果表明,鸽的HI抗体几何平均滴度(MAT)为9.00~10.0 log2,鸡的为7.13~7.63 log2,鹌鹑的为5.00~5.13 log2,鹅对灭活油乳剂苗的为5.63~6.38 log2、而对C30株活疫苗的仅为3.38log2;除了C30株活疫苗免疫鹅提供的保护率比较低(20%)外,8种油乳剂苗都能对同源或者异源强毒株的攻毒提供比较高的保护率(66.75%~100%)。研究结果表明,经典疫苗株C30与近年来从各种不同禽类分离的致病性APMV-1野毒株之间、不同禽源分离株之间的抗原性,以及各种不同禽源分离株的之间的免疫原性差异均不大。更多还原

【Abstract】 The immunization and cross-protection test was preformed in chickens,quails,geese and pigeons by using the oil-emulsion vaccines(OEV) prepared from different APMV-1 isolates from chickens,geese,pigeons,quails,peacock,thrush,Guinea fowl,and commercial attenuated vaccine strain Clone30(C30),respectively and live C30.Sera of the birds were collected and detected for hemoagglutinization inhibit(HI) antibody against NDV at different weeks post-immunization(PI),and then all the birds were challenged with the virulent NDV at PI 5 weeks.The mean average titers(MAT) of HI antibody were 9.00-10.0 log2,7.13-7.63 log2,5.00-5.13 log2,5.63-6.38 log2(C30-OEV) and 3.38 log2(live-C30) in pigeons,chickens,geese and quails,respectively.HI titers of the group vaccinated with OEV were 5.63-6.38;All the eight OEV vaccines can provide good protections(66.75%-100%) against the challenges with homogenous and heterogeneous viruses,except that live C30 can only provide the protection of 20% in geese.The results of the study demonstrated that there is no much difference in antigenicity and immunogenicity between the classic vaccine strain C30 and recent isolates of APMV-1 or among the isolates.更多还原