【摘要】 猪传染性胸膜肺炎(Porcine infectious pleuropneumonia)是由胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)引起的猪的一种呼吸道疾病。本实验以APP血清7型的一株现地分离株L25-4株为研究对象,对其分泌的ApxⅡ毒素的结构基因apxⅡA进行了全基因克隆和测序,并利用原核表达载体pGEX-6P-1在E.coli中进行了原核表达。表达产物以包涵体形式存在,包涵体蛋白经过洗涤后作为抗原用于Western-blotting免疫印迹实验,结果表明重组的ApxⅡA蛋白具有良好的免疫原性。更多还原

【Abstract】 Porcine infectious pleuropneumonia is a respiratory disease that caused by Actinobacillus pleurop-neumoniae(APP).The structure gene apxⅡA of ApxⅡtoxin that be excreted by a field isolate L25-4 of APP serotype 7 was cloned and sequenced,then apxⅡA was cloned into prokaryotic expression vector pGEX-6p-1 and expressed in E.coli BL-21(DE3).The expressed product formed inclusion,the inclusion protein was used to do Western blottingafter washing.The result showed that the recombinant ApxⅡAprotein has good immunogenicity.更多还原