【摘要】 用OPJ和OPM两组40条10碱基随机引物,对中国五大湖三角帆蚌地理群体及诸暨养殖蚌进行了随机扩增多态性DNA(RAPD)分析,其中12个引物的扩增结果具有丰富的群体多态性,多态率为55.6%～80%。群体内遗传相似度大小依次为:鄱阳湖(0.8889),太湖(0.8694),洞庭湖(0.8111),诸暨(0.7746),洪泽湖(0.7348),巢湖(0.7185)。依据群体间遗传距离指数及分子系统树,表明洞庭湖群体和洪泽湖群体亲缘关系最近,鄱阳湖群体则与巢湖群体的亲缘关系最近,并且诸暨人工养殖群体与鄱阳湖和巢湖的群体比较接近。更多还原

【Abstract】 With the random amplified polymorphic DNA (RAPD) analysis method, forty ten-base arbitrary primers of OPJ and OPM groups were used to study the genetic variability among geographic stocks of Hyriopsis cumingii from the five large lakes of China: Po-yang Lake (PY), Dong-ting Lake (DT), Tai Lake(TH), Chao Lake (CH) and Hong-ze Lake (HZ), and one cultured stock from Zhuji City (ZJ) of Zhejiang Province. The amplified results of twelve primers showed abundant polymorphy, and the percentage of polymorphy with each primer amplified varied 55.6% to 80%. The intra-stock genetic similarity indexes ranked as PY(0.888 9)>TH (0.869 4)> DT ((0.811 1))> ZJ (0.774 6)> HZ(0.734 8)> CH(0.718 5). Based on inter-stock genetic distance indexes and molecular phylogenetic tree, we can conclude that the phylogeny relation between DT and HZ stocks is closest, and the cultured stock from ZJ is relatively close to the cluster of PY and CH stocks.更多还原