【摘要】 体外构建表皮细胞 无细胞真皮活性复合皮 ,移植于裸鼠背部创面 ,并与单纯移植表皮细胞膜片相对照。术后观察创面愈合情况 ,行光镜、电镜及免疫组化检测。结果示复合皮移植后创面覆盖率高 ,外观情况良好 ,收缩程度低。组织学检测提示复合皮移植后上皮分化充分 ,胶原增生有序 ,表皮 真皮连接结构重建明显 ,提示无细胞真皮复合皮移植可改善创面愈合质量。更多还原

【Abstract】 To study the method of preparing the composite skin composed of keratinocytes and acellular dermis, and to investigate the efficacy as well as its histological changes after being grafted on wounds of athymic mice. Compared with keratinocyte sheet grafting, the wounds grafted with composite skin had better healing rate, appearance and less wound contraction rate. Moreover, histological examination revealed fully differentiated epidermis, organized proliferated collagen fibres, and distinct reconstruction of epidermis-dermis junction in composite skin group, with absence of acute immuno-rejection response. As a new kind of skin substitutes, the composite skin can improve the quality of wound healing.更多还原