【摘要】 对氨基酸的反相高效液相色谱分离和测定作了介绍。重点介绍了柱前衍生化紫外吸收检测法,这种方法的优点是仪器简单,成本低,不会引起谱峰扩展,衍生化产物较稳定,大多数可在室温下分离。列举了氨基酸注射液、蛋白水解液氨基酸含量测定结果。更多还原

【Abstract】 The separation and determination of amino acids by reversed-phase HPLC are described.The pre-column deri-vatization and UV absorption detection are described emphatically. This method is stapte and cost saving with no peak broadening. The products of derivatization are more stable and most of them can be separated under room tempera-tures. The cxperimental results for amino acids in amino acid injection and protein hydrolyte soluton are given.更多还原