【作者】 唐艳娟；

【导师】 陈槐卿；

【作者基本信息】 四川大学 ， 生物医学工程， 2006， 博士

【摘要】 引言临床上由于严重创伤、肿瘤切除、感染以及发育异常等原因而导致的骨缺损发病率较高。骨缺损是指由于外伤、感染、肿瘤切除等因素造成骨质丧失,从而在骨组织中形成间隙。较小范围的骨缺损可以由机体自身的再生得到修复,而较大间隙的骨缺损则由于成骨细胞难以越过而不能正常愈合,若不采用适当治疗则最终仅由纤维组织充填,形成骨不连。有研究表明,当长骨骨干缺损长度超过其直径的1.5～2.5倍时,机体便难以自行愈合,因而造成永久性骨不连。骨缺损的病人在骨科患者中占有很高的比例,大约有10～15%的病人需要进行骨移植治疗。如何利用现代高科技,研究用于骨缺损修复的生物材料,解除患者痛苦,是骨科临床和生物材料研究者们关注的课题。骨组织工程学是利用组织工程学原理对骨缺损进行修复或重建的一门学科。近年来,将种子细胞与支架材料复合,在体外构建生物复合材料是骨组织工程学研究的热点。理想的生物复合材料应在保证材料力学性能的同时,具有良好的生物相容性和多孔相通性,以支持细胞的生长和功能的表达,为血管、神经的生长,营养物质的输送以及代谢产物的排出提供空间,促进骨组织的生长。目前应用较广泛的种子细胞为骨髓间充质干细胞(Mesenchymal stem cells, MSCs),其作为组织工程的优秀种子细胞之一,具有很强的成骨分化潜力,在诱骨环境下能够被诱导分化成为成骨细胞,促进骨修复过程,因此被广泛应用于骨组织工程领域。而支架材料多选用生物活性材料及生物复合材料。近年来,将细胞与特更多还原

【Abstract】 The bone defect caused by many reason, such as trauma, infection is common in clinic.Bone defect is a bone lost caused by trauma, sarcoma or infection. Damaged bone will spontaneously heal provided the defect is not over a critical limit, but when the defect is critical-sized, it will be filled by fibrous tissue because the osteoblast cells can not pass the gap between two broken bones. So a proper treatment, like bone transplant, is necessary. Bone defect patients take a very important part from orthopedics patients, there are about 10~15% orthopedics patients who need bone transplant at least. The researches on the biomaterials used in bone resconstruction applying the high-techology are the hot topics focused by the clinical and biomaterial scientists.Bone tissue engineering is a subject which applying engineering and medicine principles to repair and reconstruct bone defects. Recently, researchers are mainly focusing on fabrication bioactive compounds. Ideal bioactive compounds should possess good biocompatibility and three dimension porous structure as well as assuring their mechanic properties. Only by this kind of structure can the composites support cells proliferation and differentiation, provide enough space and communicate ways for supplying adequate nutrients for blood vessels and nerves to grow in. At present, bone mesenchymal stem cells(rMSCs) have been researched and更多还原