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靶向超声造影剂无创评价组织缺血再灌注损伤的实验研究
Experimental Study on Noninvasively Evaluating Ischemia-Reperfusion Tissues Using Targeted Ultrasound Contrast Agent
【作者】 景香香；
【导师】 王志刚；
【作者基本信息】 重庆医科大学 ， 内科学， 2006， 博士
【摘要】 随着新型超声造影剂（UCA）的不断出现和超声造影新技术的飞速发展,明显提高了超声诊断心、肝、肾、脑等实质性器官病变的敏感性和特异性,但大多数超声造影剂无定向选择性（即无靶向性）,因此很难对疾病进行特异性的定性及定位诊断。本研究首先制备出用于实验的表面活性剂超声造影剂（SPMB）,并初步观察其理化性质,急性动物毒性和显像效果,在此基础上进一步将其制备成对激活的中性粒细胞（PMN）具有靶向性的超声造影剂,观察其对肾脏和心肌缺血再灌注损伤的显像效果并分析与髓过氧化物酶（MPO）的相关性,并且在体外细胞水平分析其靶向性作用的可能机制。一、表面活性剂超声造影剂的制备、安全性及显像效果的实验研究（一）表面活性剂超声造影剂的制备及其急性毒性动物实验以司盘60（Span 60）和吐温80（Tween 80）为原材料,经高温加热熔融,冷却变性后,最后在声振同时缓慢充入八氟丙烷（C3F8）气体,制备成SPMB微泡,激光粒度散射仪测量微泡的粒径分布,血球计数仪计算微泡的浓度,光镜下观察微泡的形态,并观察随时间的延长微泡粒
【Abstract】 1.Experimental study on surfactant perfluoropropane-filled microbubbles preparation, safety and visualization effectiveness1.1Experiment of surfactant perfluoropropane-filled microbubbles preparation and acute toxicity testSurfactant perfluoropropane-filled microbubbles (SPMB) were made from Span 60 and Tween 80 which were warmed up to liquate, cooled down to degeneration, then sonicated with slowly injecting perfluoropropane. The distribution of SPMB diameter was measured by laser grade scatter implement, the concentration was calculated by blood cell count and the appearance was observed by light microscope. Meanwhile the changes of SPMB diameter, concentration and appearance were observed with the storage time extension. The acute toxicity was experimented on 62 clearing mice and LD50 value was analyzed with Bliss assay method. The changes of gross anatomy and histological anatomy were observed. Results Successfully prepared SPMB microbubbles were