【作者】 邓建川；

【导师】 刘杞；

【作者基本信息】 重庆医科大学 ， 内科学， 2006， 博士

【摘要】 目的:肝再生增强因子(augmenter of liver regeneration, ALR)是近年来发现的一种促肝细胞再生的细胞因子。ALR的生物学作用主要表现在直接刺激肝细胞增殖和再生、对肝脏的保护作用、参与组织器官的形成与发育、免疫调控作用以及促肝癌细胞增殖等效应。尽管有上述重要生物学作用,但ALR究竟如何发挥生物学作用目前仍知之较少。如果能获得与ALR发生相互作用的蛋白,并且鉴定出ALR受体,将有助于深刻理解ALR的生物学功能,特别是为深入了解ALR与肝癌发生、发展的关系奠定基础。本实验拟从人肝癌细胞的cDNA噬菌体表面展示文库中筛选与人肝再生增强因子(human augmenter of liver regeneration, hALR)相互作用的特异噬菌体克隆,通过序列分析及生物学活性检测确认hALR相互作用蛋白,构建原核表达载体,为进一步鉴定ALR受体提供实验基础。方法:1.以hALR为靶蛋白,从人肝癌细胞cDNA噬菌体展示文库中筛选与hALR相互作用的特异噬菌体克隆;对获得的特异噬菌体克隆cDNA插入片段进行序列测序及生物信息学分析;利用3H-TdR掺入法测定hALR联合阳性噬菌体展示肽及单独阳性噬菌体展示肽对QGY肝癌细胞株的增殖效应。更多还原

【Abstract】 Objective: Augmenter of liver regeneration (ALR) is a newly found cytokine that could stimulate the proliferation of hepatocytes. The main biological effects of ALR include the following: promoting the proliferation and regeneration of hepatocytes, protecting the structure of liver, participating in the development of tissues or organs, immune regulation function and stimulating the proliferation of hepatoma cells. Although ALR has important and widespread biological significance, the mechanism of ALR remains unclear. If we obtain the interacting protein of ALR and identify the ALR receptor, it will be helpful to deeply understand the biological mechanism of ALR, especially the relationship between ALR and carcinogenesis of hepatocellular carcinoma. Our objective in this study was to biopan the specific phage clones interacting with hALR from cDNA library of hepatoma cell, and to confirm hALR interacting protein by sequence analysis and identification of the biological function. As the first step work, this experiment will be beneficial for further study.Methods:1. In experiment study, the specific phage clones that interact with更多还原