【作者】 吴瑛；

【导师】 赖声礼；

【作者基本信息】 华南理工大学 ， 电路与系统， 2001， 博士

【摘要】 本论文是关于毫米波细胞生物效应的基础性实验研究。课题以红细胞作为研究对象，首次结合采用生物物理学，电生理等跨学科的研究方法从各个研究层次检测低强度毫米波对红细胞生物物理特性造成的影响，包括红细胞细胞变形性和聚集性、细胞形态稳定性、膜的离子通透性以及离子通道特性等功能性指标的变化，并系统分析了实验数据和结果，初步探讨了毫米波细胞效应与理论作用机理之间可能的对应性，以及由此对研究电磁生物效应从实验可靠性角度做了深入探讨和总结。本课题的意义在于较为全面系统地揭示了毫米波的一种细胞效应，为进一步研究毫米波的生物效应及相关的临床应用提供实验基础。尤其是将膜片钳技术运用于毫米波细胞效应的研究的尝试，为从分子层次了解电磁波与细胞的作用机理提供了有效的研究方法和积累了研究经验。 本文主要由六部分内容组成： 第一章论述了本课题的研究背景、研究现状及意义，阐述了本文所用的研究方法、研究思路以及相应的研究内容。 第二章研究了低强度毫米波对红细胞生物力学特性的影响，本章采用血流动力学的检测方法，对离体血液在低强度毫米波照射下血流动力特性的改变进行测量，依据生物力学理论，推算出红细胞的变形性及聚集性等力学特性指标的变化。 第三章研究了毫米波对红细胞形态稳定性的影响。一方面，采用细胞溶血实验检测细胞溶血脆性的大小，定量比较红细胞在毫米波照射前后形态稳定性的变化；另一方面，用扫描电镜观察红细胞的外形在毫米波照射前后的改变。 第四章研究了毫米波对红细胞膜离子通透性的影响。本章对细胞膜对阴离子和阳离子的通透能力在毫米波照射刺激下的变化分别进行研究，测试离子选择了较有用重要生理功能性的NO^2-及Ca²⁺。 第五章采用膜片钳技术研究了毫米波照射对红细胞膜的钙激活钾通道的影响。实验中使用了inside-out单通道电流记录方法，对红细胞在低强度毫米波照射前后通道的开放概率、开放时间及通道电导等指标的改变作对照分析。 第六章是关于毫米波细胞效应机理的初步探讨及进一步研究的设想。对于毫米波细胞效应研究在理论及实验方面要深入进展所面临的关键问题作了充更多还原

【Abstract】 The paper is the experimental research about the cellular biological effects of millimeter wave. The influence of low intensity millimeter wave on the biophysical property of erythrocytes was studied by biophysical and electrophysiological methods from different research level. The studied biophysical property of erythrocytes was included the deformability and aggregating ability、the stability of cellular configuration、 ionic transport of membrane and ionic channel etc. The experimental results and the possible connection with respecting theories were well analyzed and discussed. The reliability and standardization in experimental research about the biological effects of electromagnetic wave were discussed also. The meaning of the full investigation is provided the experimental basis for further research about the biological effects of millimeter wave. Especially more effective studied way and experience were gained from that the technology of patch clamp was used in the field of research about the cellular effects of millimeter.The paper were composed of six parts of contents:The relative background 、 present status and meaning of the task as well as investigating methods and contents were introduced in the first chapter.In chapter two, the influence of low-density millimeter wave on the biomechanical property of erythrocytes were studied using the rheological methods. Depending on the biomechanical theory, the variety of deformability and aggregating ability, which caused by millimeter wave, were finded in radiated intro blood samples.In chapter three, the variety of configurational stability of radiated erythrocytes by millimeter wave were discovered from two aspects. One quantitative index is the osmotic frangibility and anther intuitionistic result is the morphological change of cell.The variety of the ionic transports of cellular membrane that caused by the millimeter wave was described in chapter four. The transport of anion and cation such as NO^2- and Ca²⁺ between membrane of erythrocytes were investigated separately.In chapter five, the technology of patch clamp was used to investigate the variety of Ca²⁺-activated-K⁺ channel of the erythrocytes radiated by millimeter. The更多还原