黄斑康对血脂异常小鼠光感受器影响的实验研究

【作者】 王丽英；

【导师】 曾庆华；

【作者基本信息】 成都中医药大学 ， 中医眼科学， 2005， 博士

【摘要】 目的:观察实验性血脂异常C57BL小鼠光感受器等眼组织的病理改变,探讨该动物模型与年龄相关性黄斑变性(AMD)的相关性以及黄斑康对该模型的治疗机理的探讨。 方法:首先采用高脂饲料喂养方法,建立C57BL小鼠血脂异常动物模型。3月龄C57BL小鼠根据体重随机分为3组。第1组为3月龄普食组12只,喂食1W,处死;第2组为8月龄普食组12只;第3组为8月龄高脂组48只。第2、3组喂养5月后,用单纯随机抽样法从第3组中抽取12只,与第2组同时处死,采血检测血脂、血液流变学和血清丙二醛(MDA),同时摘取眼球做病理切片,采用Masson染色和免疫组织化学方法染色,光镜和电镜观察。确认模型成功后,将造模成功的小鼠再随机分为4、5、6组,每组12只,普通饮食。第4组为阴性对照组,生理盐水灌胃4W;第5组为阳性药物对照组,西药复方灌胃4W;第6组为黄斑康治疗组,黄斑康灌胃4W。然后处死动物作上述各指标检测。用SPSS11.5统计软件包,对实验数据结果进行统计学处理。 结果:第3组抽样检测结果与第1、2组比较,其体重、血清胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)水平、血液流变学指标、血清丙二醛(MDA)水平均升高(P<0.01或P<0.05);视网膜色素上皮(RPE)层和外核层变薄,细胞数量减少(P<0.01),二者超微结构改变;免疫组织化学指标异常(P<0.01),上述结果与AMD改变相似,模型成功。对模型组进行4W治疗后,黄斑康组体重减轻,血清TC、LDL-C、MDA水平降低,免疫组化指标下调(或上调),与阴性对照组和阳性药物对照组比较,有显著性差异(P<0.01或P<0.05),光感受器、RPE细胞超微结构明显改善。更多还原

【Abstract】 OBJECTIVE: To observe the pathological change of photoceptor and other tissues of eye in experimental dyslipidemia C57BL mice and investigate the relationship between the dyslipidemia model and Age-related Macular Degeneration(AMD) and explore the therapeutical effect of the Chinese recipe Huangbankang on the dyslipidemia model at the same time. METHODS: The dyslipidemia model copyed by feeding fat dietary in C57BL mice. The three-month-old mice were randomized to three groups: three-month-old chow-fed group, eight-month-old chow-fed group and eight-month-old high-fat-fed group. The three-month-old group (twelve mice) sacrificed one week later; The eight-month-old group(twelve mice) sacrificed five months later; The eight-month-old high-fat-fed group (forty eight mice) fed with high fat diet for five mouths and then twelve mice were selected randomly and sacrificed. All of serum lipid, hemorheology, malondialdehyde(MDA) index and eyeball structure were measured. Pathological section of the mice eyeballs were observed under light microscope and transmission electron microscope (TEM) by immunohistochemistry staining and Masson staining. After dyslipidemia model were confirmed, the remained eight-month-old dyslipidemia model were randomized to three groups: the negative control group, positive medicine control group and Huangbankang treated group. Each group had twelve mice and fed by normal diet for four weeks and then all the mice were executed and the mentioned indexes above were detected. The results were analized according to the SPSS for windows 11.5 software. RESULTS: The body weight, total cholesterol (TC ), low density lipoprotein-cholesterol (LDL-C), hemorheological index and serum MDA in dyslipidemia model group were higher than the three-month-old chow-fed group and the更多还原