电针对转基因鼠类风湿性关节炎HLA-DR₄基因调控的研究

【作者】 赵征宇；

【导师】 梁繁荣；

【作者基本信息】 成都中医药大学 ， 针灸推拿学， 2004， 博士

【摘要】 目的：以转基因鼠为研究对象，运用现代生物技术，从免疫学，遗传学等角度深入系统地探讨电针对类风湿性关节炎易感基因调控机制。 方法：采用人类HLA－DR₄β1基因转基因小鼠，转基因阴性小鼠和非转基因小鼠胶原性关节炎为疾病模型，以电针为主要治疗方法，将大于5周龄的小鼠随机分成电针治疗组、甲氨喋呤对照组和模型对照组，运用分子生物学、遗传学、免疫学、细胞学和病理学等多种手段和技术，从动物个体、组织、细胞、分子及基因水平，研究电针治疗RA的特点和环节，并阐明其作用机理。 结果：①电针具有明显的抗炎、镇痛作用；②电针可以抑制T淋巴细胞和NK细胞的活化；③电针在调控类风湿性关节炎易感基因的表达中有一定的作用。 结论：电针通过调控类风湿性关节炎易感基因的表达，影响MHCⅡ类分子的抗原递呈功能达到对CIA小鼠的治疗目的。更多还原

【Abstract】 Objective: To research the heredity and immunity mechanism of electro-acupuncture on rheumatoid arthritis(RA) by observing the effect of electro-acupuncture on HLA-DR4 transgenic RA animal model.Methods: The type II collagen-induced arthritis(CIA) in HLA-DR4β 1 transgenic mice, negative transgenic mice and non-transgenic mice was taken as research subjective. Every kinds of mice was random divided into electro-acupuncture group, methotrexate group and model contrast group. The feature and mechanisms of electro-acupuncture on RA were detected by using RT-PCR, Northern blot, Southern blot, dot blot, radio-immunoassay and pathologic methods.Result: firstly, electro-acupuncture could significantly attenuate inflammation and increase the pain threshold of CIA mice. Secondly, electo-acupuncture could inhibit the activities of T cell and NK cell. Thirdly, electro-acupuncture could regulate the expression of RA predisposing genes at some degree.Conclusion: The electro-acupuncture therapy treated CIA mice trough regulating the expression of RA predisposing genes and influencing the antigen presentation of MHCII moleculars.更多还原