【作者】 王德先；

【导师】 杨更亮；

【作者基本信息】 河北大学 ， 分析化学， 2003， 博士

【摘要】 全文共分三章。 在第一章综述里，介绍了分子烙印技术的原理、方法、应用和展望，介绍了高效液相色谱在天然、合成和生化药物分析方面的具体应用。 第二章，分子烙印技术。本章里，分别以己酮可可碱，咖啡因，茶碱单组分，以及己酮可可碱-茶碱和咖啡因-茶碱混合物(摩尔比均为2:1)为烙印分子，考察了单烙印分子和混合烙印分子高聚物的色谱特性；通过沉淀聚合法合成了咖啡因烙印高聚物微球，比较了咖啡因分别在烙印和空白高聚物微球上的吸附，利用Scatchard分析考察了咖啡因在烙印高聚物微球上的结合特性；合成了氨基安替比林分子烙印高聚物固定相。并利用前流分析和静态分析法研究了氨基安替比林、安替比林和吡唑酮单组分以及氨基安替比林与安替比林的竞争吸附等温线。 第二章，高效液相色谱用于天然和基因重组抗癌药物分离分析。本章建立了共溶剂超临界流体提取方法结合高效液相色谱从天然植物西伯利亚高丽参中提取五加甙-D。考察了不同的萃取压力、流体中水含量对萃取效果的影响；建立了分析和循环制备色谱从西伯利亚高丽参中制备五加甙-D的优化方法；利用高效前流分析法(HPFA)测定了紫苏子醇与人血清白蛋白(HSA)的结合常数；采用C8反相液相色谱法分离和纯化人重组白细胞介素-18，考察了不同因素对IL-18色谱特性的影响，纯化的IL-18回收率为87％；利用毛细管电泳法，分离和测定了中药首乌中的蒽醌类有效成分—大黄素甲醚，大黄酚、芦荟大黄素、大黄素、大黄酸，并考察了缓冲溶液pH以及有机改性剂(乙腈)对分离的影响。更多还原

【Abstract】 There are three chapters in this thesis. In Chapter I, the principle of molecularimprinted polymers(MIPs), polymerization methods, application of this technique and itsfuture developments were introduced. Meanwhile, the applications of HPLC on theanalysis of anti-cancer drugs were also described.Chapter II: Molecular imprinted polymers. First, molecular imprinted polymers (MIPs) by using single template(pentoxifhylline, caffeine and theophylline) and mixed-templates (pentoxifhylline-theophylline and caffeine-theophylline) were investigated in this chapter; Second, Caffeine molecular imprinted uniform microgel spheres were prepared by precipitation polymerization method, comparison of caffeine adsorption on molecular imprinted and blank microgel spheres was made and Scatchard analysis was used to investigate the binding properties of caffeine on imprinted microgel spheres. Third, the molecular imprinted technique was applied for the preparation aminoantipyrine imprinted polymer stationary phase. The adsorption isotherms of aminoantipyrine, antipyrine, pyrazolone and the competitive adsorption isotherms of aminoantipyrine and antipyrine on the imprinted stationary phase were determined using rectangular pulse frontal analysis and static method.Chapter III: Separation and analysis of natural and genetic anti-cancer drugs by HPLC. In this chapter, supercritical fluid extraction (SFE) with co-solvent scheme for the extraction of Acanthoside-D from Acanthopanax senticosus was developed. Different pressure and water content in the fluid were investigated to see their effects on extraction results; Second, the optimum operating conditions were experimentally determined in the analytical and preparative recycle chromatography for the extraction and preparation ofacanthoside-D from Acanthopanax Senticosus. Apart from the above study, high-performance frontal analysis (HPFA) was used for protein binding study of perillyl alcohol (POH) enantiomers to human serum albumin (HSA) , a RP-HPLC method was developed for the separation and purification of recombinant human interleukin-18(IL-18) on C8 column, and a CE method for the separation and determination of the active anthraquinone components, physcion, chrysophanol, aloe-emodin, emodin, and rhein in Chinese herb Polygnum multiflorum Thunb. were developed.更多还原