几丁质广泛存在于大多数真菌细胞壁内,是这些生物体不可缺少的结构成分.苹果炭疽病是一种严重危害苹果生长成熟的由围小丛壳菌(Glomerella cingulata)引起的传染病,而利用几丁质酶能够分解几丁质的性质,可以作为一种抑制苹果炭疽病原菌的新方法.本实验室分离筛选到一株高产几丁质酶的黏质沙雷氏菌L15-2,该菌株在含有1%胶体几丁质、0.3%K2HPO4、0.3%KH2PO4、0.05%MgSO4、0.05%CaCl2、0.001%FeSO4 的产酶培养基中37℃培养4天后产酶,粗酶液经过DEAE Sepharose Fast Flow和Sephadex G—100,电泳检测得到电泳纯的几丁质酶。该酶的性质特征测定结果表明,L15-2 几丁质酶稳定性较好:在60℃、pH4-10范围内较稳定:各种金属离子对酶活力影响不同,Fe2+、Zn2+抑制作用明显,而Ba2+、Mn2+对酶活力有一定的促进作用。我们对该酶抑制苹果炭疽病原菌的效果进行了初步研究表明,该几丁质酶几乎可以完全抑制病原菌的生长.
【英文论文摘要】
Chitin can be found in the most of epiphytes' cell wall. It is the structure element of them. Apple anthracnose is a serious disease that caused by Glomerella cingulata. Chitinase can be a new way to inhibit the epiphyte for the characteristic of chitinase that can decompose chitin. A strain of Serratia marcesens that can produce chitinase was researched. The optimal fermentation medium composed of 1%chitin, 0.3%K2HPO4, 0.3%KH2PO4, 0.05%MgSO4, 0.05%CaCl2 and 0.001%FeSO4. After being purified by DEAE Sepharo...
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