Pollen tube pathway transgenesis is one of the most promising transgenic methods for the improvement of the tree peony (Paeonia suffruticosa Andr. ) in the present. In this paper, the stigma was cut off the style in different time after supplementary pollinated to pistil of peony cultivar Fengdanbai. When the fruit expands enough big, the development conditions are investigated. It shows that the pollen tube pathway has formed after 1h of pollination. After 1h of pollination, transgenic manipulation can han...