Objective: Study the biotechnology and form the best condition to culture the stems of Rubia cordifolia. Research the best way to induce callus of Rubia cordifolia and gain the medicinal matter. Method: Using the biotechnology of plant tissue culture to culture stems of Rubia cordifolia on different medium, and induce them to form the whole plants. The stems, leaves and roots were put into induction media to induce callus. Result and conclusion: The induction of callus was best on MS medium with the additio...