CoTAPc-Fe3O4 magnetic nanoparticle composites were prepared,and laccase was immobilized on it by glutaraldehyde.The factors to influence the enzyme immobilization such as glutaraldehyde concentration、temperature and pH of immobilization、BSA concentration、given amount of enzyme were studied.The optimum condition were as follows: glutaraldehyde concentration was 5%,immobilization temperature was 0 ℃,immobilization was pH 7 and BSA concentration was 2.5 mg/mL and given amount of enzyme was 2 mL.The immobilized...
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