Purpose To establish a method for the determination of resibufogenin in Liushen pills by ion suppression chromatography.MethodsA Kromasil C18 column was used.The mobile phase consisted of acetonitrile-0.5% acetic acid(52∶48).The UV detection wavelength was 299 nm.ResultsThis method showed a good linear relationship.The average recovery of resibufogenin was 99.3% with RSD of 2.25%.ConclusionThe method was simple,reliable,sensitive and reproducible.It can be used for the quality control of Liushen pills.