Twenty-eight SSR primers were designed according to 32 SSR specific loci of Eriobotrya japonica isolated by 5′ anchor primer(s).These SSR primers with their corresponding 5′anchor degenerated primer(s) were applied in amplification of five cultivar genomes of Eriobotrya japonica.Twenty-seven primer pairs could efficiently amplify the genomes.Based on the characteristics of SSR products,24 primer pairs could be used in further Eriobotrya japonica genome research.