The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhong- shuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from Zhongshuan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B...
【英文摘要】
The fatty acid elongase 1 (FAE1) genes of Brassic napus were cloned from two cultivars, i.e. Zhong- shuan No. 9 with low erucic acid content, and Zhongyou 821 with high erucic acid content, using the degenerate PCR primers. The sequence analysis showed that there was no intron within the FAE1 genes. The FAE1 genes from Zhongyou 821 contained a coding sequence of 1521 nucleotides, and those cloned from Zhongshuan No. 9 contained a 1517 bp coding sequence. Alignment of the FAE1 sequences from Brassica rapa, B...
【基金】
the National Natural Science Foundation of China (Grant No. 30471099);
Development Plan of the State Key Fundamental Research of China (Grant No. 2006CB101600);
the National High Technology and Development Program of China (Grant No. 2006AA10A113)
【更新日期】
2007-07-12
【分类号】
S565.4;Q943.2
【正文快照】
Brassica napus (AACC, 2n=4x=38) is an amphidiploid species originating from a spontaneous hybridization of Brassica rapa (AA, 2n=2x=20) and Brassica oleracea (CC, 2n=2x=18)[1]. Since the amphidiploid nature, B. napus, contains multiple copies in genome fo
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