Ultra-cryopreservation of plant germplasm materials has been proven to be a potentially ideal method for preserving plant germplasm for long-term stable conservation.For a successful ultra-cryopreservation,the key is to apply an appropriate pretreatment to the plant material prior to storing in liquid nitrogen(LN) to avoid freezing injury;the effect of ultra-cryopreservation is determined by different factors such as material characteristics,pre-culture before freezing,cryoprotectant,falling temperature and...
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