Objective To determine the content of adenosine in Radix Eupalorri Chinense Linn by RPHPLC.Methods Samples were analyzed on a DiamonsilTM C18 column(250 mm×4.6 mm,5 μm) using CH3OH-NaH2PO4·Na2HPO4(12∶88)as mobile phase.The flow rate was 1.0 mL·min-1 and the detection wavelength 260 nm.Results The method exhibited good linearity from 0.0396 to 0.6336 μg.The average recovery for adenosine was 99.5% and RSD 1.69%(n=6).Conclusion The method could be used to determine adenosine content in Radix Eupalorri Chinen...