The mixed amino acids from Hibiscus syriacus L.leaves have been determined,which was based on fluorescence quenching effect by adding amino acid into the reaction system of chitosan and ninhydrin.The linear range for determination of amino acid was 0.5～3.0 mmol/L,the limit of detection was 0.1 mmol/L.The total content of free amino acid in Hibiscus L.leaves was 10.18%,coefficient of variation was 1.3% and the recoveries of glutamic acid were in the range of 102.5%～94.3%.
【更新日期】
2007-06-27
【分类号】
O657.3
【正文快照】
木槿(Hibiscus syriacus L.)是集药用、食用、观赏、绿化、工业价值于一身的木本植物[1,2],木槿叶中含丰富的营养成分与无机元素[4],测定木槿叶中的氨基酸对木槿的综合开发利用有一定的意义。氨基酸与茚三酮反应生成ruhemann’s purple(RP),溶液呈蓝紫色,利用分光光度计在565 n
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