The effects of the concentration of MS micro salts,2,4-dichlorophenoxyacetic acid(2,4-D),naphthalene acetic acid(NAA),prolin and adenine on callus formation and embryogenesis were investigated using orthogonal design with immature cotyledon of Chinese onion.The results showed that the role of concentration of NAA,proline or adenine could promote callus formation.The concentration of MS mecro salts possessed obvious effects on somatic enbryogenesis of Chinese onion.The category and concentration of the hormo...