The aim of the research was to discuss the genetic relationships between Piper methysticum,Pepper and other wild species in Pepper genus.With 28 germplasms including 6 materials of P.methysticum,21 materials of cultivated and wild Pepper,1 material of Peperomia pellucida belonged to different genus as tested materials,DNA was extracted from leaves and premiers with good band-type and high polymorphism and resolution were screened from 64 pairs of primers for AFLP amplification and the clustering analysis wa...