The seed,bud and stem of Dahlia pinnata F1 were selected as explants, MS was used as the fundamental culture medium including 5 kinds of different media,4 kinds of generation reproduce media,4 kinds of rooting media,and 4 kinds of ground substances for plantlets-transplating.The preliminary conclusion was: the suitable inducing medium for bud and stem was MS + 6-BA 0.5 mg/L+NAA 0.2 mg/L,the generation medium was MS + 6-BA 1.0 mg/L+ NAA 0.2 mg/L+ B9 0.1 mg/L,the rooting medium was 1/2MS + NAA 0.1 mg/L and th...