The tested Trichoderma strains were first thermal-kept for 24 h at 25,35,40,45,50 ℃,then cultivated at 28 ℃ for 6 d to 7 d for screening fire-resistant bacterial strain.The result showed that the fireresistant biocontrol strain could produce more stress-tolerant protein after thermal shock,with great expression.The eluting peak of single protein was obtained from fireresistant stress-tolerant protein through the DEAE-32 and G-100 column chromatographye.In SDS-PAGE electrophoresis it was found the fireresist...
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