The orthogonal design was adopted in an experiment to determine the processing conditions for extraction of chlorogenic acid from the shell meal of sunflower(Helianthus annuus L.),which can be added to animal feed for its antibiotic activity.The optimum extraction parameters appeared to be material/water ratio 1 ∶10,circumfluence temperature 60~oC,pH 6 and maximum absorbing wavelength of the extractant 326 nm.The substances extracted showed some antibiotic activity against Escherichia coli,Staphylococcus au...