<正>Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specifi...
【英文摘要】
Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific m...
【更新日期】
2006-04-07
【分类号】
Q943.2
【正文快照】
1 IntrodUCtion Mierosatellites or simple sequenee repeats(SSRs)are widely distributed throughout diverse genomes(Tautz and Renz,1984).Inter一simplesequeneerepeat(ISSR) 15 derived from SSR,whieh amPlifies speeifieally the region between two mierosatellit