Optimum medium composition in lipase production via Penicillium camembertii Thom PG-3 fermentation was investigated using Response Surface Methodology(RSM).The impact of initial medium constituents on lipase production was studied by Fractional Factorial Design(FFD),revealing that both ammonium secondary phosphate and fat-free soybean meal have a substantial influence on lipase production.In our work,the greatest response area was estimated by the design of steepest ascent approach,and the optimum concentra...